基于Label-free技术分析牛卵泡蛋白质组分及关键调控蛋白

doi:10.11843/j.issn.0366-6964.2019.05.009

Abstract

Abstract:

The purpose of this study was to investigate changes in proteome expression and screen key regulatory proteins during bovine follicular development, and protein components of GCs(granulesa cells)were analyzed by label-free quantitative proteomics technology.DF(dominant follicles)and SF(subordinate follicles)during the bovine estrous cycle were collected, GCs were separated, total proteins were extracted, proteins were digested with trypsin and analyzed by LC-MS/MS, the expression of proteins in DF and SF were analyzed after database retrieval, and key regulatory proteins were screened by bioinformatics method. In this study, 3 409 proteins were successfully identified from 30 321 peptide fragments (FDR ≤ 0.01), of which 2 895 were DF proteins and 3 102 were SF proteins, 259 differentially expressed proteins were identified (Fold change>2,P<0.05), 17 differential expressed proteins might be related to the process of bovine follicular dominance, and SERPINB2 might regulate bovine follicular atresia.The results enriched the studies on DF and SF proteome and laid a foundation for further studies on follicular atresia and dominance theory by identify differential expressed proteins and specific expression proteins.

CLC Number:

S823.2

HAO Qingling, JING Jiongjie, HOU Shuning, XU Dongmei, ZHAO Chengping, ZHU Zhiwei, Lü Lihua, LI Pengfei. Proteomic Analysis of Follicular Components and Key Regulatory Proteins in Bovine Based on Label-free Technology[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 983-992.

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Proteomic Analysis of Follicular Components and Key Regulatory Proteins in Bovine Based on Label-free Technology

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